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magnetic microbeads miltenyi biotec  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec magnetic microbeads miltenyi biotec
    Magnetic Microbeads Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 663 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd31+microbeads/CD31+MicroBeads%2C+mouse/pm42501329-276-41-43
    Average 96 stars, based on 663 article reviews
    magnetic microbeads miltenyi biotec - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Pericyte Loss Reprogrammes Capillary Endothelium and Drives White Matter Injury in Small Vessel Disease
    Article Snippet: Following removal of myelin, debris, and red blood cells, the pellet was resuspended in 80 μL PB buffer and incubated with 10 μL FcR Blocking Reagent (Miltenyi Biotec, 5240403597) for 10 min at 4 °C. .. CD31-positive vascular cells were labelled by addition of 10 μL CD31 MicroBeads (Miltenyi Biotec, 130-097-418) and incubation for 15 min at 4 °C in the dark. .. To enrich the vascular fraction, the suspension was passed through pre-wetted pre-separation filters (Miltenyi Biotec, 9221100219) attached to magnetic columns attached to an OctoMACS separator.

    Article Title: Endocytosis at the mouse blood brain barrier is elevated during sleep
    Article Snippet: .. 10 μL of CD31 MicroBeads (Miltenyi Biotec, 130-097-418) were added, mixed gently, and incubated on ice for 15 minutes. .. The MiniMACS Separator (Miltenyi Biotec, 130-042-102) was set up by placing an MS Column in the magnetic field.

    Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies
    Article Snippet: In total, 20 μL of non-neuronal cell biotin-antibody cocktail (Miltenyi #130-115-390) was added to the cell suspension and incubated at 2–8 °C. .. After 5 min, 20 μL of anti-biotin MicroBeads (Miltenyi #130-115-390) and 10 μL of CD31 MicroBeads (Miltenyi #130-097-418) were added to the cell suspension and incubated at 2–8 °C for 12 min. A final volume of 500 μL of cell mixture was allowed to flow through the LS columns. ..

    Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies.
    Article Snippet: In total, 20 μL of non-neuronal cell biotin-antibody cocktail (Miltenyi #130-115-390) was added to the cell suspension and incubated at 2–8 ◦C. .. After 5 min, 20 μL of anti-biotin MicroBeads (Miltenyi #130-115-390) and 10 μL of CD31 MicroBeads (Miltenyi #130-097-418) were added to the cell suspension and incubated at 2–8 ◦C for 12 min. A final volume of 500 μL of cell mixture was allowed to flow through the LS columns. ..

    Article Title: MARCH2 prevents doxorubicin-induced cardiomyopathy by stabilizing NR1H2 and promoting clearance of apoptotic cardiomyocytes.
    Article Snippet: Hearts were excised, minced into approximately 2-mm fragments, and enzymatically dissociated using a commercial enzyme kit (Miltenyi Biotec, Cat No. 130-110-201) according to the manufacturer's protocol to generate a single-cell suspension. .. The single-cell suspension was filtered through a 70-μm strainer to remove cellular aggregates, followed by incubation with CD31 MicroBeads (Miltenyi Biotec, Cat No. 130- 097-418) for 20 minutes (bead-to-cell ratio: 10 μL MicroBeads per 1×107 total cells). .. After washing, ECs were isolated via LD Column (Miltenyi Biotec, Cat No. 130-042-901) using AR TI CL E IN P RE SS magnetic separation as specified by the manufacturer.

    Suspension:

    Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies
    Article Snippet: In total, 20 μL of non-neuronal cell biotin-antibody cocktail (Miltenyi #130-115-390) was added to the cell suspension and incubated at 2–8 °C. .. After 5 min, 20 μL of anti-biotin MicroBeads (Miltenyi #130-115-390) and 10 μL of CD31 MicroBeads (Miltenyi #130-097-418) were added to the cell suspension and incubated at 2–8 °C for 12 min. A final volume of 500 μL of cell mixture was allowed to flow through the LS columns. ..

    Article Title: Astro-Versus Microglia-Enriched Transcriptomes from Aged Atxn2 -CAG100-Knockin Mice Suggest Underlying Pathology of RNA Processing at Ribosomes, and Possibly at U-Bodies.
    Article Snippet: In total, 20 μL of non-neuronal cell biotin-antibody cocktail (Miltenyi #130-115-390) was added to the cell suspension and incubated at 2–8 ◦C. .. After 5 min, 20 μL of anti-biotin MicroBeads (Miltenyi #130-115-390) and 10 μL of CD31 MicroBeads (Miltenyi #130-097-418) were added to the cell suspension and incubated at 2–8 ◦C for 12 min. A final volume of 500 μL of cell mixture was allowed to flow through the LS columns. ..

    Article Title: MARCH2 prevents doxorubicin-induced cardiomyopathy by stabilizing NR1H2 and promoting clearance of apoptotic cardiomyocytes.
    Article Snippet: Hearts were excised, minced into approximately 2-mm fragments, and enzymatically dissociated using a commercial enzyme kit (Miltenyi Biotec, Cat No. 130-110-201) according to the manufacturer's protocol to generate a single-cell suspension. .. The single-cell suspension was filtered through a 70-μm strainer to remove cellular aggregates, followed by incubation with CD31 MicroBeads (Miltenyi Biotec, Cat No. 130- 097-418) for 20 minutes (bead-to-cell ratio: 10 μL MicroBeads per 1×107 total cells). .. After washing, ECs were isolated via LD Column (Miltenyi Biotec, Cat No. 130-042-901) using AR TI CL E IN P RE SS magnetic separation as specified by the manufacturer.

    Magnetic Cell Separation:

    Article Title: IDH2 deficiency enhances ferroptosis-induced vascular inflammation and remodeling in diabetes.
    Article Snippet: .. CD31 MicroBeads (cat no. 130-097-418) and CD45 MicroBeads (cat no. 130-052-301) used for MACS were obtained from Miltenyi Biotec, Inc. (Bergisch Gladbach, Germany). ..

    Labeling:

    Article Title: Endothelial ADGRF5(GPR116) governs vascular adaptation required for sustained thermogenic remodeling of brown adipose tissue
    Article Snippet: Cell suspensions were filtered through 100 μm and 40 μm strainers, subjected to red blood cell lysis (ACK buffer) for 1min, and incubated with Fc receptor blocking reagent (Miltenyi). .. CD31 + cells were labeled with CD31 microbeads (Miltenyi, #130-097-418) and isolated using LS columns. ..

    Isolation:

    Article Title: Endothelial ADGRF5(GPR116) governs vascular adaptation required for sustained thermogenic remodeling of brown adipose tissue
    Article Snippet: Cell suspensions were filtered through 100 μm and 40 μm strainers, subjected to red blood cell lysis (ACK buffer) for 1min, and incubated with Fc receptor blocking reagent (Miltenyi). .. CD31 + cells were labeled with CD31 microbeads (Miltenyi, #130-097-418) and isolated using LS columns. ..

    Single Cell:

    Article Title: MARCH2 prevents doxorubicin-induced cardiomyopathy by stabilizing NR1H2 and promoting clearance of apoptotic cardiomyocytes.
    Article Snippet: Hearts were excised, minced into approximately 2-mm fragments, and enzymatically dissociated using a commercial enzyme kit (Miltenyi Biotec, Cat No. 130-110-201) according to the manufacturer's protocol to generate a single-cell suspension. .. The single-cell suspension was filtered through a 70-μm strainer to remove cellular aggregates, followed by incubation with CD31 MicroBeads (Miltenyi Biotec, Cat No. 130- 097-418) for 20 minutes (bead-to-cell ratio: 10 μL MicroBeads per 1×107 total cells). .. After washing, ECs were isolated via LD Column (Miltenyi Biotec, Cat No. 130-042-901) using AR TI CL E IN P RE SS magnetic separation as specified by the manufacturer.



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    Miltenyi Biotec cd31 microbead kit
    A ) Schematic diagram of the stepwise differentiation of iPSCs into CECs. B ) Bright-field images: day 0; iPSCs, day 5; embryoid bodies, day 8; differentiating cells. The <t>CD31-positive</t> endothelial cells were isolated (day 14) and expanded from the heterogeneous population using the CD31 MicroBead Kit. Representative brightfield images of CD31-negative and CD31-positive cells at day 20. Scale bars = 100 μm. C ) Quantitative RT-PCR showing enrichment of CEC markers in the CD31 + enriched cell populations in both HR and ISO cultures. Data are presented as mean ± SEM, n=3, unpaired t-test. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    Image Search Results


    A ) Schematic diagram of the stepwise differentiation of iPSCs into CECs. B ) Bright-field images: day 0; iPSCs, day 5; embryoid bodies, day 8; differentiating cells. The CD31-positive endothelial cells were isolated (day 14) and expanded from the heterogeneous population using the CD31 MicroBead Kit. Representative brightfield images of CD31-negative and CD31-positive cells at day 20. Scale bars = 100 μm. C ) Quantitative RT-PCR showing enrichment of CEC markers in the CD31 + enriched cell populations in both HR and ISO cultures. Data are presented as mean ± SEM, n=3, unpaired t-test. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: bioRxiv

    Article Title: Oxidative Stress Susceptibility, Complement Dysregulation, and Metabolic Reprogramming in CFH Y402H Patient-Derived Choriocapillaris Endothelial Cells

    doi: 10.64898/2026.06.18.733111

    Figure Lengend Snippet: A ) Schematic diagram of the stepwise differentiation of iPSCs into CECs. B ) Bright-field images: day 0; iPSCs, day 5; embryoid bodies, day 8; differentiating cells. The CD31-positive endothelial cells were isolated (day 14) and expanded from the heterogeneous population using the CD31 MicroBead Kit. Representative brightfield images of CD31-negative and CD31-positive cells at day 20. Scale bars = 100 μm. C ) Quantitative RT-PCR showing enrichment of CEC markers in the CD31 + enriched cell populations in both HR and ISO cultures. Data are presented as mean ± SEM, n=3, unpaired t-test. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: On day 14, after cell treatment with TrypLe Express (Life Technologies, 12604013), CD31-positive cells were isolated from a single cell suspension with the CD31 MicroBead Kit (Miltenyi Biotec, 130-091-935) according to the manufacturer’s protocol.

    Techniques: Isolation, Quantitative RT-PCR

    CECs were stained for choriocapillaris endothelial cell markers: CD31, RGCC, CA4, PLVAP (red). Nuclei were counterstained with Hoechst (blue). Positive staining was assessed by comparing to a secondary antibody-only control, scale bars = 50 μm.

    Journal: bioRxiv

    Article Title: Oxidative Stress Susceptibility, Complement Dysregulation, and Metabolic Reprogramming in CFH Y402H Patient-Derived Choriocapillaris Endothelial Cells

    doi: 10.64898/2026.06.18.733111

    Figure Lengend Snippet: CECs were stained for choriocapillaris endothelial cell markers: CD31, RGCC, CA4, PLVAP (red). Nuclei were counterstained with Hoechst (blue). Positive staining was assessed by comparing to a secondary antibody-only control, scale bars = 50 μm.

    Article Snippet: On day 14, after cell treatment with TrypLe Express (Life Technologies, 12604013), CD31-positive cells were isolated from a single cell suspension with the CD31 MicroBead Kit (Miltenyi Biotec, 130-091-935) according to the manufacturer’s protocol.

    Techniques: Staining, Control